Pde8b^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pde8b^{tm1b(KOMP)Wtsi}
• Name: phosphodiesterase 8B; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6511098
• Gene: Pde8b Location: Chr13:95160962-95386844 bp, - strand Genetic Position: Chr13, 49.22 cM
• Alliance: Pde8b^{tm1b(KOMP)Wtsi} page
• IMPC: Pde8b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:302971
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 95243939 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 95244837. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s).Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:302971)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pde8b Mutation: 57 strains or lines available

References

• Original: J:302971 Leal LF, et al., Pde8b haploinsufficiency in mice is associated with modest adrenal defects, impaired steroidogenesis, and male infertility, unaltered by concurrent PKA or Wnt activation. Mol Cell Endocrinol. 2021 Feb 15;522:111117
• All: 1 reference(s)