Fermt2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Fermt2^{tm1c(EUCOMM)Wtsi}
• Name: fermitin family member 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6507948
• Synonyms: Kind2^flox
• Gene: Fermt2 Location: Chr14:45696252-45767575 bp, - strand Genetic Position: Chr14, 22.97 cM
• Alliance: Fermt2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Fermt2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:302197
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 45712791 of Chromosome 14 upstream of the critical exons 5-6 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exons at position 45714360. The critical exons 5-6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons 5-6. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:302197)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fermt2 Mutation: 32 strains or lines available

References

• Original: J:302197 Ruiz-Ojeda FJ, et al., Active integrins regulate white adipose tissue insulin sensitivity and brown fat thermogenesis. Mol Metab. 2021 Jan 7;45:101147
• All: 4 reference(s)