Ddx4^tm1(cre)Dcp
Targeted Allele Detail

Summary

• Symbol: Ddx4^tm1(cre)Dcp
• Name: DEAD box helicase 4; targeted mutation 1, David C Page
• MGI ID: MGI:6505285
• Synonyms: Mvh^Cre-mOrange
• Gene: Ddx4 Location: Chr13:112734867-112789009 bp, - strand Genetic Position: Chr13, 63.87 cM
• Alliance: Ddx4^tm1(cre)Dcp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:198777
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Ddx4^tm1(cre)Dcp expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ddx4 (MGI:102670)

• Mutation details: The sequence encoding a cre-mOrange fusion protein was inserted into the Ddx4 (Mvh) locus by homologous recombination with a targeting vector in ES cells. Part of exon 1 and intron 1 were replaced by the cre-mOrange sequence. A PGK-neo selection marker also was inserted. The mOrange fluorescent reporter in the construct does not appear to be functional. (J:198777)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ddx4 (mouse) Summary of all recombinase alleles driven by Ddx4. Alliance mouse Ddx4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ddx4 Mutation: 58 strains or lines available

Notes

The version of this allele that does not contain the neomycin selection cassette, Ddx4^tm1.1Dcp, has been analyzed.

References

• Original: J:198777 Hu YC, et al., Tumor suppressor gene Rb is required for self-renewal of spermatogonial stem cells in mice. Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12685-90
• All: 1 reference(s)