Nras^tm1.1Kshn
Targeted Allele Detail

Summary

• Symbol: Nras^tm1.1Kshn
• Name: Nras proto-oncogene, GTPase; targeted mutation 1.1, Kevin Shannon
• MGI ID: MGI:6469907
• Synonyms: Nras^{LSL-G12D/C181S}
• Gene: Nras Location: Chr3:102965643-102975230 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Nras^tm1.1Kshn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:289386
• Parent Cell Line: V26.2 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert an FRT-flanked neomycin resistance (neo) cassette downstream of exon 5. Endogenous exon 5 was modified to harbor a guanine to cytosine mutation at codon 181, resulting in a cysteine to serine mutation (C181S). This targeting vector then was used to retarget Nras^tm1Tyj inserting C181S in cis to the existing G12Dmutation. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:289386)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nras Mutation: 40 strains or lines available

References

• Original: J:289386 Zambetti NA, et al., Genetic disruption of N-RasG12D palmitoylation perturbs hematopoiesis and prevents myeloid transformation in mice. Blood. 2020 May 14;135(20):1772-1782
• All: 1 reference(s)