Igs2^{tm1(CAG-CD59)Bga}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(CAG-CD59)Bga}
• Name: intergenic site 2; targeted mutation 1, Bin Gao
• MGI ID: MGI:6469895
• Synonyms: ihCD59, LSL-hCD59
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(CAG-CD59)Bga} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237074
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Igs2^{tm1(CAG-CD59)Bga} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CD59 (966)

  Cd59a	(MGI:109177)
  Cd59b	(MGI:1888996)

• Mutation details: Site-specific PhiC31 integrase-mediated transgenesis is used to insert a chicken beta-actin promoter with a CMV enhancer (CAG promoter), followed by a loxP-flanked stop cassette and the entire open reading frame of the human CD59 gene into the intergenic site two locus. This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice. (J:101977, J:237074)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 69 strains or lines available

Notes

TARGATT mice carry the H11 allele ( Igs2^tm3.1Luo) or H11P3; a unique sequence from the promoter of the yeast his3 gene followed by three shortened tandem attP sites and one FRT5 site. One founder mouse carried the correctly targeted transgenic insertion of the construct into the first and second attP sites of the locus.

References

• Original: J:237074 Feng D, et al., Cre-inducible human CD59 mediates rapid cell ablation after intermedilysin administration. J Clin Invest. 2016 Jun 01;126(6):2321-33
• All: 5 reference(s)