Igk^{tm3.1(VRCO1)Nemz}
Targeted Allele Detail

Summary

• Symbol: Igk^{tm3.1(VRCO1)Nemz}
• Name: immunoglobulin kappa chain complex; targeted mutation 3.1, David Nemazee
• MGI ID: MGI:6459233
• Synonyms: VRC01^gL
• Gene: Igk Location: Chr6:67532620-70703738 bp, + strand Genetic Position: Chr6, 30.89 cM, cytoband F1-pter

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:259005
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector was constructed using a loxP-flanked neomycin cassette and the HIV antibody germline-reverted VRC01 sequence including the mature LCDR3 (light chain complementarity determining region 3) to replace the J cluster from Igk. Cre-mediated recombination removed the floxed neo cassette. (J:259005)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igk Mutation: 33 strains or lines available

References

• Original: J:259005 Abbott RK, et al., Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens. Immunity. 2018 Jan 16;48(1):133-146.e6
• All: 4 reference(s)