Prdm10^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Prdm10^{tm1d(EUCOMM)Hmgu}
• Name: PR domain containing 10; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6458945
• Synonyms: Prdm10^delta
• Gene: Prdm10 Location: Chr9:31191834-31293019 bp, + strand Genetic Position: Chr9, 16.77 cM
• Alliance: Prdm10^{tm1d(EUCOMM)Hmgu} page
• IMPC: Prdm10 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294860
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31235437 of Chromosome 9 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 31237409. Exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) tm1c allele was created by flp recombinase expression in mice carrying the tm1a allele. Exon 5 was deleted through subsequent cre-mediated recombination. (J:294860)

Expression

In Mice Carrying this Mutation:

3 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm10 Mutation: 81 strains or lines available

References

• Original: J:294860 Han BY, et al., Global translation during early development depends on the essential transcription factor PRDM10. Nat Commun. 2020 Jul 17;11(1):3603
• All: 1 reference(s)