Ano10^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ano10^{tm1b(EUCOMM)Wtsi}
• Name: anoctamin 10; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6456850
• Gene: Ano10 Location: Chr9:122004958-122123440 bp, - strand Genetic Position: Chr9, 72.94 cM
• Alliance: Ano10^{tm1b(EUCOMM)Wtsi} page
• IMPC: Ano10 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:294632
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 122088304 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 122091060. The critical exon(s) is/are thus flanked by loxP sites. A reporter knockout mouse was created through subsequent cre-mediated recombination. (J:294632)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ano10 Mutation: 31 strains or lines available

References

• Original: J:294632 Petkovic M, et al., TMEM16K is an interorganelle regulator of endosomal sorting. Nat Commun. 2020 Jul 3;11(1):3298
• All: 1 reference(s)