Col1a1^{tm1(tetO-GFP/RNAi:Shroom3)Bmu}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-GFP/RNAi:Shroom3)Bmu}
• Name: collagen, type I, alpha 1; targeted mutation 1, Barbara Murphy
• MGI ID: MGI:6451687
• Synonyms: Shroom3-RNAi
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-GFP/RNAi:Shroom3)Bmu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220042
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A transgenic construct containing the following elements was inserted into the 3' UTR of the collagen 1a1 gene: the tetO tetracycline responsive element, a fusion of GFP fluorescent protein gene sequence and shRNA sequence targeting the Shroom3 gene, and a hygromycin resistance gene cassette. (J:220042)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 161 strains or lines available

References

• Original: J:220042 Menon MC, et al., Intronic locus determines SHROOM3 expression and potentiates renal allograft fibrosis. J Clin Invest. 2015 Jan;125(1):208-21
• All: 2 reference(s)