Aqp5^tm2.1Niba
Targeted Allele Detail

Summary

• Symbol: Aqp5^tm2.1Niba
• Name: aquaporin 5; targeted mutation 2.1, Nick Barker
• MGI ID: MGI:6450543
• Synonyms: Aqp5-2A-eGFP
• Gene: Aqp5 Location: Chr15:99488909-99492710 bp, + strand Genetic Position: Chr15, 56.13 cM
• Alliance: Aqp5^tm2.1Niba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292580
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: Sequence for the 2A self-cleaving peptide, the EGFP fluorescent marker gene and a loxP site flanked neomycin resistance gene cassette were inserted immediately before the stop codon. The neo cassette was removed through subsequent cre-mediated recombination. This allele expresses the endogenous peptide and the inserted EGFR peptide. (J:292580)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aqp5 Mutation: 21 strains or lines available

References

• Original: J:292580 Tan SH, et al., AQP5 enriches for stem cells and cancer origins in the distal stomach. Nature. 2020 Feb;578(7795):437-443
• All: 1 reference(s)