Kcne2^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kcne2^{tm1b(EUCOMM)Wtsi}
• Name: potassium voltage-gated channel, Isk-related subfamily, gene 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:6416412
• Gene: Kcne2 Location: Chr16:92089277-92095017 bp, + strand Genetic Position: Chr16, 53.55 cM
• Alliance: Kcne2^{tm1b(EUCOMM)Wtsi} page
• IMPC: Kcne2 gene page

Mutation origin

• Mutant Cell Line: EPD0156_2_F10
• Germline Transmission: Earliest citation of germline transmission: J:169366
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 92093176 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 92095205. The critical exon(s) is/are thus flanked by loxP sites. Cre expression occurs without flp expression produced a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:169366)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcne2 Mutation: 10 strains or lines available

References

• Original: J:169366 MouseBook^TM, Information obtained from MouseBook^TM, Medical Research Council Mammalian Genetics Unit, Harwell, UK. Unpublished. 2005-2013
• All: 1 reference(s)