Satb1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Satb1^{tm1c(EUCOMM)Hmgu}
• Name: special AT-rich sequence binding protein 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6415623
• Synonyms: Satb1^floxed
• Gene: Satb1 Location: Chr17:52043215-52140318 bp, - strand Genetic Position: Chr17, 26.81 cM
• Alliance: Satb1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Satb1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235693
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 52111059 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 52111946. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:235693)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Satb1 Mutation: 46 strains or lines available

References

• Original: J:235693 Tesone AJ, et al., Satb1 Overexpression Drives Tumor-Promoting Activities in Cancer-Associated Dendritic Cells. Cell Rep. 2016 Feb 23;14(7):1774-86
• All: 6 reference(s)