Dicer1^tm2.1Glo
Targeted Allele Detail

Summary

• Symbol: Dicer1^tm2.1Glo
• Name: dicer 1, ribonuclease type III; targeted mutation 2.1, Guillermina Lozano
• MGI ID: MGI:6364600
• Synonyms: Dicer^S1836D
• Gene: Dicer1 Location: Chr12:104654001-104718211 bp, - strand Genetic Position: Chr12, 54.83 cM, cytoband E-F1
• Alliance: Dicer1^tm2.1Glo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278360
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutation: Nucleotide substitutions
• Mutation details: This allele was generated from a vector that contains two TC>GA mutations (in exon 24 and 26) that create serine to aspartic acid phosphomimetic mutations that lead to a constitutively active enzyme: p.S1712D and p.S1836D. The vector has the potential to generate three different alleles (the two single mutations and one double mutation), depending on the location of the cross-over breakpoints during homologous recombination. The other allele that was created is Dicer1^tm1.1Glo. The neomycin reistance gene cassette that was inserted into intron 24, was removed through subsequent cre-mediated recombination. (J:278360)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dicer1 Mutation: 96 strains or lines available

References

• Original: J:278360 Aryal NK, et al., Constitutive Dicer1 phosphorylation accelerates metabolism and aging in vivo. Proc Natl Acad Sci U S A. 2019 Jan 15;116(3):960-969
• All: 1 reference(s)