Tnfrsf9^em3Ygch
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tnfrsf9^em3Ygch
• Name: tumor necrosis factor receptor superfamily, member 9; endonuclease-mediated mutation 3, Yi-Guang Chen
• MGI ID: MGI:6306269
• Synonyms: Tnfrsf9^-
• Gene: Tnfrsf9 Location: Chr4:151004612-151030561 bp, + strand Genetic Position: Chr4, 81.52 cM
• Alliance: Tnfrsf9^em3Ygch page

Mutation origin

• Strain of Origin: NOD.B10Sn-Idd9.3^C57BL/10SnJ/1106MrkTacJ

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: Zinc finger nuclease (ZFN)-mediated genome editing was used in NOD.Idd9.3 zygotes to target the second coding exon of the Tnfrsf9 gene (target sequence CCCTCCAAGTACCTTctccaGCATAGGTGGACAGCCGAA). Progeny were sequenced at the locus targeted by the ZFN to map the insertion. Founder line 24 contains a two base-pair insertion (aa) at the target site, resulting in a premature stop codon. (J:274998)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnfrsf9 Mutation: 46 strains or lines available

References

• Original: J:274998 Forsberg MH, et al., Combined congenic mapping and nuclease-based gene targeting for studying allele-specific effects of Tnfrsf9 within the Idd9.3 autoimmune diabetes locus. Sci Rep. 2019 Mar 13;9(1):4316
• All: 1 reference(s)