Palb2tm1.1Bix
Targeted Allele Detail
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Symbol: |
Palb2tm1.1Bix |
Name: |
partner and localizer of BRCA2; targeted mutation 1.1, Bing Xia |
MGI ID: |
MGI:6295979 |
Synonyms: |
Palb2CC6, Palb2CC6 (LKK-AAA) |
Gene: |
Palb2 Location: Chr7:121706485-121732203 bp, - strand Genetic Position: Chr7, 65.42 cM
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Alliance: |
Palb2tm1.1Bix page
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Allele Type: |
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Targeted (Conditional ready) |
Mutation: |
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Nucleotide substitutions
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Mutation details: This allele was derived from the Palb2tm1.1Dli allele, which has a loxP site in intron 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP iste in intron 3. A mutation was introduced into part of exon 2 coding for the CC (coiled-coil) domain using site-directed mutagenesis. The TTGAAAAAG>GCAGCAGCT nucleotide substitution changes peptide sequence LeuLysLys to AlaAlaAla in the encoded protein (p.(L24_K26delinsAAA)). This amino-acid trio represents residues 15-18 of the 33 aa CC motif and the substitution negatively affects binding to Brca1. The neo cassette was removed through flp-mediated recombination.
(J:267154)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Palb2 Mutation: |
50 strains or lines available
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Original: |
J:267154 Simhadri S, et al., Male fertility defect associated with disrupted BRCA1-PALB2 interaction in mice. J Biol Chem. 2014 Aug 29;289(35):24617-29 |
All: |
3 reference(s) |
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