Pcdhg^em12Rwb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Pcdhg^em12Rwb
• Name: protocadherin gamma cluster; endonuclease-mediated mutation 12, Robert W Burgess
• MGI ID: MGI:6285787
• Synonyms: 3R1
• Gene: Pcdhg Location: unknown Genetic Position: Chr18, Syntenic

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele was generated through shotgun CRISPR/Cas9 editing using pooled sgRNAs that targeted each variable exon of the Pcdhg cluster and this allele was found to have a fusion between exons A5 and B8, and additional junctions between A1 and A4, A12 and A9, and B6 and A6 with an insertion of a transposable element and sequence from Anp32a exons 4-7 3-prime of exon A5, which, in sum, left only the variable exons C3, C4, and C5 intact.
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcdhg Mutation: 13 strains or lines available

References

• Original: J:285314 Garrett AM, et al., CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4. PLoS Genet. 2019 Dec;15(12):e1008554
• All: 2 reference(s)