Kif17^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kif17^{tm1b(EUCOMM)Wtsi}
• Name: kinesin family member 17; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6272012
• Synonyms: Osm-3/Kif17^tm1b(Bjc)
• Gene: Kif17 Location: Chr4:137989562-138029284 bp, + strand Genetic Position: Chr4, 70.07 cM, cytoband D3
• Alliance: Kif17^{tm1b(EUCOMM)Wtsi} page
• IMPC: Kif17 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242150
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 138001771 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 138002912. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:242150)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kif17 Mutation: 36 strains or lines available

References

• Original: J:242150 Lewis TR, et al., Cos2/Kif7 and Osm-3/Kif17 regulate onset of outer segment development in zebrafish photoreceptors through distinct mechanisms. Dev Biol. 2017 May 15;425(2):176-190
• All: 3 reference(s)