In(7Peg3-Usp29)1Jkim
Targeted Allele Detail

Summary

• Symbol: In(7Peg3-Usp29)1Jkim
• Name: inversion, Chr 7, Joomyeong Kim 1; inversion Chr 7, Joomyeong Kim 1
• MGI ID: MGI:6258925
• Synonyms: Peg3^Inv
• Gene: In(7Peg3-Usp29)1Jkim Location: unknown Genetic Position: Chr7, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:245745
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Inversion
• Mutation details: This allele was created by crossing mice carying the Usp29^tm2Jkim allele with cre-expressing mice (Tg(Zp3-cre)93Knw). Usp29^tm2Jkim was created as follows: a loxP site was inserted into intron 1 of Peg3 upstream of the Peg3-DMR (differentially methylated region). An FRT site flanked neomycin resistance gene cassette (in the same transcriptional direction as the targeted gene) and a second loxP site, in the opposite orientation of the first loxP site, were inserted into intron 1 of downstream neigbor Usp29, with which Peg3 shares a bidirectional promoter. Because of the opposite orientation of the two loxP sites, cre-mediated recombination inverts the ~4 kb Peg3-DMR region including the first exons of Peg3 and Usp29. This creates two fusion genes: one gene with Peg3 exon 1 followed by Usp29 exons 2-9, and the other gene with Usp29 exon 1 followed by Peg3 exons 2-9. Subsequent flp-mediated recombination removed the neo cassette, which would interfere with the transcription of the Usp29-Peg3 fusion gene. (J:245745)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any In(7Peg3-Usp29)1Jkim Mutation: 0 strains or lines available

References

• Original: J:245745 Kim J, et al., Inversion of the imprinting control region of the Peg3 domain. PLoS One. 2017;12(7):e0181591
• All: 2 reference(s)