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Transgene Detail
Symbol: Tg(KRT5-Terf2)PMBlas
Name: transgene insertion PM, Maria A Blasco
MGI ID: MGI:6258221
Synonyms: PM K5-Terf2
Transgene: Tg(KRT5-Terf2)PMBlas  Location: unknown  Genetic Position: ChrX, Syntenic
Strain of Origin:  (C57BL/6 x CBA)F1
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(KRT5-Terf2)PMBlas expresses 1 gene
Mutation detailsThe full-length mouse Terf2 cDNA was placed under control of the 5' regulatory region of the bovine keratin K5 promoter, which targets TERF2 to basal cells and stem cells of the epidermis. Three lines were generated and analyzed (PM, PN and PO); a fourth line (PL) had undetectable levels of transgene expression and was not studied further. In the PM line, the transgene is linked to the X chromosome and is selectively silenced in transgenic females. The PM line (males only) showed the highest levels of Terf2 mRNA expression in tail and back skin (~59 times greater than wild-type controls), followed by the PO line (~23 times greater) and the PN line (~12 times greater). Western blot analysis revealed significantly elevated TERF2 protein levels in primary keratinocytes derived from PM and PO transgenic mice. Combined immunofluorescence and telomeric Q-FISH analysis showed that the overexpressed TERF2 protein localized to telomeres. (J:102653)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 8 anatomical structures
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Original:  J:102653 Munoz P, et al., XPF nuclease-dependent telomere loss and increased DNA damage in mice overexpressing TRF2 result in premature aging and cancer. Nat Genet. 2005 Oct;37(10):1063-71
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.21
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