Rora^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rora^{tm1c(EUCOMM)Wtsi}
• Name: RAR-related orphan receptor alpha; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6256736
• Synonyms: Rora^fl
• Gene: Rora Location: Chr9:68561068-69295528 bp, + strand Genetic Position: Chr9, 37.45 cM
• Alliance: Rora^{tm1c(EUCOMM)Wtsi} page
• IMPC: Rora gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263583
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 69268555 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 69269394. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:263583)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rora Mutation: 43 strains or lines available

References

• Original: J:263583 Oliphant CJ, et al., MHCII-mediated dialog between group 2 innate lymphoid cells and CD4(+) T cells potentiates type 2 immunity and promotes parasitic helminth expulsion. Immunity. 2014 Aug 21;41(2):283-95
• All: 18 reference(s)