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Efcab3tm1.1Osb
Targeted Allele Detail
Summary
Symbol: Efcab3tm1.1Osb
Name: EF-hand calcium binding domain 3; targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
MGI ID: MGI:6195014
Gene: Efcab3  Location: Chr11:104954418-105008363 bp, + strand  Genetic Position: Chr11, Syntenic
Alliance: Efcab3tm1.1Osb page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:234235
Parent Cell Line:  EGR-G101 (ES Cell)
Strain of Origin:  C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 104996692 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 104997583. The critical exon(s) is/are thus flanked by loxP sites. Cre mediated recombination without flp expression produced a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:234235)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Efcab3 Mutation:  26 strains or lines available
References
Original:  J:234235 Miyata H, et al., Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7704-10
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory