Fus^tm3(FUS)Emcf
Targeted Allele Detail

Summary

• Symbol: Fus^tm3(FUS)Emcf
• Name: fused in sarcoma; targeted mutation 3, Elizabeth MC Fisher
• MGI ID: MGI:6193741
• Gene: Fus Location: Chr7:127565276-127581204 bp, + strand Genetic Position: Chr7, 69.87 cM
• Alliance: Fus^tm3(FUS)Emcf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169366
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The RP24-297F14 bacterial artificial chromosome (BAC), from the RPCI-24 mouse genomic library derived from a C57BL/6J male mouse, was modified by replacement of the sequence from the translation initiation site through the 3' untranslated region (3'-UTR) by the orthologous human sequence. A FRT site-flanked neomycin selection cassette was inserted ~1 kb downstream of the humanized 3'-UTR. This modified BAC was then used to target the endogenous mouse gene. (J:169366)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fus Mutation: 57 strains or lines available

References

• Original: J:169366 MouseBook^TM, Information obtained from MouseBook^TM, Medical Research Council Mammalian Genetics Unit, Harwell, UK. Unpublished. 2005-2013
• All: 1 reference(s)