Lag3^tm1Daav
Targeted Allele Detail

Summary

• Symbol: Lag3^tm1Daav
• Name: lymphocyte-activation gene 3; targeted mutation 1, Dario AA Vignali
• MGI ID: MGI:6188309
• Synonyms: Lag3^L-YFP
• Gene: Lag3 Location: Chr6:124881324-124888668 bp, - strand Genetic Position: Chr6, 59.17 cM
• Alliance: Lag3^tm1Daav page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260063
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: Exon 7, containing the CP cleavage site, was flanked by loxP sites. An IRES-YFP was inserted between the Lag3 stop codon and the pA. A FRT-flanked neomycin selection cassette was inserted just past the pA. Upon cre-mediated removal of exon 7, these mice are expected to lack cell surface expression of LAG3 and thus cannot mediate signaling but continue to release soluble protein. (J:260063)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lag3 Mutation: 41 strains or lines available

References

• Original: J:260063 Zhang Q, et al., LAG3 limits regulatory T cell proliferation and function in autoimmune diabetes. Sci Immunol. 2017 Mar 31;2(9)
• All: 3 reference(s)