Malat1^tm1.1Svdi
Targeted Allele Detail

Summary

• Symbol: Malat1^tm1.1Svdi
• Name: metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA); targeted mutation 1.1, Sven Diederichs
• MGI ID: MGI:6188018
• Gene: Malat1 Location: Chr19:5845718-5852700 bp, - strand Genetic Position: Chr19, 4.34 cM
• Alliance: Malat1^tm1.1Svdi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260021
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The complete 6,982 bp of coding sequence, including 250 nucleotides upstream of the transcriptional start site and 321 nucleotides following the 3'-end of the transcript, was flanked by loxP sites. A FRT-flanked neomycin cassette was inserted downstream of the coding sequence but upstream of the second loxP site. Cre-mediated recombination removed the entire floxed region. qRT-PCR confirmed absence of transcripts in various organs. (J:260021)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Malat1 Mutation: 8 strains or lines available

References

• Original: J:260021 Eissmann M, et al., Loss of the abundant nuclear non-coding RNA MALAT1 is compatible with life and development. RNA Biol. 2012 Aug;9(8):1076-87
• All: 4 reference(s)