Mapk8ip1^tm4.1Rjd
Targeted Allele Detail

Summary

• Symbol: Mapk8ip1^tm4.1Rjd
• Name: mitogen-activated protein kinase 8 interacting protein 1; targeted mutation 4.1, Roger J Davis
• MGI ID: MGI:6155707
• Synonyms: Mapk8ip1^deltaJBD
• Gene: Mapk8ip1 Location: Chr2:92214021-92231608 bp, - strand Genetic Position: Chr2, 50.99 cM, cytoband E1
• Alliance: Mapk8ip1^tm4.1Rjd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261382
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector introduced point mutations (GCTCAACCT to CGGCCGCGG) in exon 3 resulting in a leucine to glycine substitution at amino acid 160 (L160G), an asparagine to arginine change at amino acid 161 (N161R), and a leucine to glycine change at amino acid 162 (L162G), creating the delta JBD mutation. In addition, a floxed neomycin resistance cassette was introduced in intron 3. Cre-mediated recombination excised the selection cassette. The delta JBD mutation results in a defective JNK-binding domain. (J:261382)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapk8ip1 Mutation: 31 strains or lines available

References

• Original: J:261382 Kant S, et al., A Protein Scaffold Coordinates SRC-Mediated JNK Activation in Response to Metabolic Stress. Cell Rep. 2017 Sep 19;20(12):2775-2783
• All: 2 reference(s)