Piezo1^tm3.2Apat
Targeted Allele Detail

Summary

• Symbol: Piezo1^tm3.2Apat
• Name: piezo-type mechanosensitive ion channel component 1; targeted mutation 3.2, Ardem Patapoutian
• MGI ID: MGI:6154896
• Synonyms: Piezo1GOF
• Gene: Piezo1 Location: Chr8:123208437-123278068 bp, - strand Genetic Position: Chr8, 71.31 cM
• Alliance: Piezo1^tm3.2Apat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261124
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site and FRT flanked neo cassette were inserted in intron 44, a synthetic Poly A signal, F3 flanked puro cassette and loxP site were inserted after exon 51, and a repeat of exons 45-51 with a CG7742-7743>AC (p.R2482H) mutation in exon 51 were inserted via homologous recombination. Recombinase mediated recombination removed the neo and puro cassettes, exons 45-51and the synthetic PolyA sequence allowing expression of the mutated exon 51. (J:261124)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Piezo1 Mutation: 89 strains or lines available

Notes

ES cell= TaconicArtemis

References

• Original: J:261124 Ma S, et al., Common PIEZO1 Allele in African Populations Causes RBC Dehydration and Attenuates Plasmodium Infection. Cell. 2018 Apr 5;173(2):443-455.e12
• All: 4 reference(s)