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Targeted Allele Detail
Symbol: Sh3bp2tm1c(KOMP)Wtsi
Name: SH3-domain binding protein 2; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID: MGI:6150886
Synonyms: Sh3bp2fl
Gene: Sh3bp2  Location: Chr5:34525838-34563641 bp, + strand  Genetic Position: Chr5, 17.89 cM
Mutant Cell Line:  EPD0129_3_C05
Germline Transmission:  Earliest citation of germline transmission: J:243612
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion     Vector: L1L2_Bact_P
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 34556459 of Chromosome 5 upstream of the critical exons 6 and 7 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 6 and 7 at position 34557639. The critical exons 6 and 7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:243612)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Sh3bp2 Mutation:  23 strains or lines available
Original:  J:243612 Matsumoto Y, et al., RANKL coordinates multiple osteoclastogenic pathways by regulating expression of ubiquitin ligase RNF146. J Clin Invest. 2017 Apr 03;127(4):1303-1315
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.15
The Jackson Laboratory