Actb^em1Akas
Endonuclease-mediated Allele Detail

Summary

• Symbol: Actb^em1Akas
• Name: actin, beta; endonuclease-mediated mutation 1, Anna Kashina
• MGI ID: MGI:6117098
• Synonyms: Actbc-g
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^em1Akas page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9-mediated gene editing was used to introduce five point mutations into the native mouse beta-actin gene, altering it to encode gamma-actin protein. In homozygous mice, no beta-actin protein is produced, and gamma-actin is expressed under the control of the endogenous beta-actin promoter. (J:256857)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 55 strains or lines available

References

• Original: J:256857 Vedula P, et al., Diverse functions of homologous actin isoforms are defined by their nucleotide, rather than their amino acid sequence. Elife. 2017 Dec 15;6:e31661
• All: 1 reference(s)