Tead1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tead1^{tm1c(KOMP)Wtsi}
• Name: TEA domain family member 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6111824
• Synonyms: Tead1^F
• Gene: Tead1 Location: Chr7:112278563-112505991 bp, + strand Genetic Position: Chr7, 58.8 cM, cytoband F2
• Alliance: Tead1^{tm1c(KOMP)Wtsi} page
• IMPC: Tead1 gene page

Mutation origin

• Mutant Cell Line: EPD0207_2_H11
• Germline Transmission: Earliest citation of germline transmission: J:254424
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 112438143 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 112438977. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:254424)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tead1 Mutation: 275 strains or lines available

References

• Original: J:254424 Liu R, et al., Tead1 is required for maintaining adult cardiomyocyte function, and its loss results in lethal dilated cardiomyopathy. JCI Insight. 2017 Sep 7;2(17)
• All: 7 reference(s)