Adgrf5^tm1Vlcg
Targeted Allele Detail

Summary

• Symbol: Adgrf5^tm1Vlcg
• Name: adhesion G protein-coupled receptor F5; targeted mutation 1, Velocigene
• MGI ID: MGI:5924942
• Synonyms: Gpr116^-
• Gene: Adgrf5 Location: Chr17:43671342-43770448 bp, + strand Genetic Position: Chr17, 19.73 cM
• Alliance: Adgrf5^tm1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243171
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: DNA sequence comprising the beta-galactosidase (lacZ) reporter gene and a loxP site flanked neomycin resistance gene driven by human UBC and bacterial EM7 promoters, replaced a 32 kbp region of the target gene spanning exons 4 to 21. Severely reduced transcription from this knock-out allele was shown by qRT-PCR. No lacZ activity was detected by either qRT-PCR or enzymatic assays. (J:243171)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adgrf5 Mutation: 75 strains or lines available

References

• Original: J:243171 Niaudet C, et al., Gpr116 Receptor Regulates Distinctive Functions in Pneumocytes and Vascular Endothelium. PLoS One. 2015;10(9):e0137949
• All: 2 reference(s)