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Rnaseh2btm1c(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Rnaseh2btm1c(EUCOMM)Wtsi
Name: ribonuclease H2, subunit B; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID: MGI:5911393
Synonyms: Rnaseh2bFLOX
Gene: Rnaseh2b  Location: Chr14:62332068-62372996 bp, + strand  Genetic Position: Chr14, 33.19 cM, cytoband C3
Mutation
origin
Mutant Cell Line:  EPD0087_4_A02
Germline Transmission:  Earliest citation of germline transmission: J:186986
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 62353057 of Chromosome 14 upstream of the critical exon 5 (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 5 at position 62353921. The critical exon 5 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:186986)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rnaseh2b Mutation:  27 strains or lines available
References
Original:  J:186986 Hiller B, et al., Mammalian RNase H2 removes ribonucleotides from DNA to maintain genome integrity. J Exp Med. 2012 Jul 30;209(8):1419-26
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/04/2020
MGI 6.14
The Jackson Laboratory