Sucnr1^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sucnr1^{tm1b(EUCOMM)Wtsi}
• Name: succinate receptor 1; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5910687
• Synonyms: Sucnr1 KO
• Gene: Sucnr1 Location: Chr3:59989290-59994987 bp, + strand Genetic Position: Chr3, 29.17 cM, cytoband D
• Alliance: Sucnr1^{tm1b(EUCOMM)Wtsi} page
• IMPC: Sucnr1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238908
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 59993055 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 59995201. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). (J:238908)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sucnr1 Mutation: 27 strains or lines available

References

• Original: J:238908 McCreath KJ, et al., Targeted disruption of the SUCNR1 metabolic receptor leads to dichotomous effects on obesity. Diabetes. 2015 Apr;64(4):1154-67
• All: 3 reference(s)