Flicr^em2Doi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Flicr^em2Doi
• Name: Foxp3 regulating long intergenic noncoding RNA; endonuclease-mediated mutation 2, Christophe Benoist and Diane Mathis
• MGI ID: MGI:5908597
• Gene: Flicr Location: ChrX:7439839-7447255 bp, + strand Genetic Position: ChrX, 3.41 cM, cytoband A1.1
• Alliance: Flicr^em2Doi page

Mutation origin

• Strain of Origin: NOD

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Using an sgRNA (5'-GCAAGACTGGCTTCAGGCCTG-3') targeting the exon 3 splice acceptor site with CRISPR/Cas9, an allele with a 12 bp deletion of that site was created. Exon 3 has high homology to the human ortholog and its splice site is conserved. Only one progeny was born. (J:241346)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Flicr Mutation: 1 strain or line available

References

• Original: J:241346 Zemmour D, et al., Flicr, a long noncoding RNA, modulates Foxp3 expression and autoimmunity. Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3472-E3480
• All: 1 reference(s)