Prdx6^tm4.1Abf
Targeted Allele Detail

Summary

• Symbol: Prdx6^tm4.1Abf
• Name: peroxiredoxin 6; targeted mutation 4.1, Aron B Fisher
• MGI ID: MGI:5908015
• Synonyms: H26A Prdx6, H26A Prdx6 knock-in, Prdx6 H26A
• Gene: Prdx6 Location: Chr1:161067682-161078780 bp, - strand Genetic Position: Chr1, 69.75 cM
• Alliance: Prdx6^tm4.1Abf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: EAP6 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The mutant allele contains point mutations changing the nucleotides corresponding to positions 76 and 77 of the mRNA sequence (NM_007453.4) from CA to GC, replacing the CAC histidine codon at amino acid position 26 to GCC, an alanine codon (H26A). This histidine, with S32 and D140, constitute the phospholipase A2 (PLA2) catalytic site. A frt site-flanked neomycin resistance cassette introduced between exons 1 and 2 by the targeting vector has been deleted by FLP recombinase, leaving a single frt site. The H26A mutant protein has lost phospholipase A 2 (PLA2) activity, but retains lysophosphatidylcholine acyl transferase (LPCAT) activity; as for peroxidase activity, it retains the ability to reduce short chain hydroperoxides, but cannot reduce phospholipid hydroperoxides. (J:94077, J:231458)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prdx6 Mutation: 52 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 2 reference(s)