Sirt1^tm3.1Mcby
Targeted Allele Detail

Summary

• Symbol: Sirt1^tm3.1Mcby
• Name: sirtuin 1; targeted mutation 3.1,Michael W McBurney
• MGI ID: MGI:5903422
• Synonyms: sirt1^ER
• Gene: Sirt1 Location: Chr10:63154784-63174814 bp, - strand Genetic Position: Chr10, 32.57 cM
• Alliance: Sirt1^tm3.1Mcby page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241633
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph, Inducible)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Mutation details: A knock-in targeting vector was designed to fuse the region encoding the hormone-binding domain (amino acids 281-599 ) of the mouse estrogen receptor alpha to exon 9 of the C-terminal end of the SIRT1 coding region, removing all but the 3' 130 bases of the 1.8 kb 3' Sirt1 UTR. Esr1 contains a point mutation (G525R) that confers selective binding to 4-hydroxy-tamoxifen (4OHT). A loxP-flanked PGK puromycin cassette is located downstream of exon 9. Cre-mediated recombination removed the floxed puro cassette. Sirtuin activity in untreated mouse embryo fibroblasts is 30% of wild-type, increasing 130% in tamoxifen treated cells. (J:241633)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sirt1 Mutation: 78 strains or lines available

References

• Original: J:241633 Clark-Knowles KV, et al., Reversible modulation of SIRT1 activity in a mouse strain. PLoS One. 2017;12(3):e0173002
• All: 2 reference(s)