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Sirt1tm3.1Mcby
Targeted Allele Detail
Nomenclature
Symbol: Sirt1tm3.1Mcby
Name: sirtuin 1; targeted mutation 3.1,Michael W McBurney
MGI ID: MGI:5903422
Synonyms: sirt1ER
Gene: Sirt1  Location: Chr10:63319005-63339035 bp, - strand  Genetic Position: Chr10, 32.57 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:241633
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Hypomorph, Inducible)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA knock-in targeting vector was designed to fuse the region encoding the hormone-binding domain (amino acids 281-599 ) of the mouse estrogen receptor alpha to exon 9 of the C-terminal end of the SIRT1 coding region, removing all but the 3' 130 bases of the 1.8 kb 3' Sirt1 UTR. Esr1 contains a point mutation (G525R) that confers selective binding to 4-hydroxy-tamoxifen (4OHT). A loxP-flanked PGK puromycin cassette is located downstream of exon 9. Cre-mediated recombination removed the floxed puro cassette. Sirtuin activity in untreated mouse embryo fibroblasts is 30% of wild-type, increasing 130% in tamoxifen treated cells. (J:241633)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sirt1 Mutation:  51 strains or lines available
References
Original:  J:241633 Clark-Knowles KV, et al., Reversible modulation of SIRT1 activity in a mouse strain. PLoS One. 2017;12(3):e0173002
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory