Opn3^{tm2b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Opn3^{tm2b(EUCOMM)Wtsi}
• Name: opsin 3; targeted mutation 2b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5897190
• Synonyms: Opn3^lacz
• Gene: Opn3 Location: Chr1:175489987-175520198 bp, - strand Genetic Position: Chr1, 81.66 cM, cytoband H3
• Alliance: Opn3^{tm2b(EUCOMM)Wtsi} page
• IMPC: Opn3 gene page

Mutation origin

• Mutant Cell Line: EPD0197_3_E01
• Germline Transmission: Earliest citation of germline transmission: J:227078
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 175492614 of Chromosome 1 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 175493673. The critical exon 2 is thus flanked by loxP sites. Cre expression occur without flp expression generates a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:227078)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Opn3 Mutation: 23 strains or lines available

References

• Original: J:227078 Buhr ED, et al., Neuropsin (OPN5)-mediated photoentrainment of local circadian oscillators in mammalian retina and cornea. Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):13093-8
• All: 5 reference(s)