Ctbp2^tm1.2Sud
Targeted Allele Detail

Summary

• Symbol: Ctbp2^tm1.2Sud
• Name: C-terminal binding protein 2; targeted mutation 1.2, Thomas C Sudhof
• MGI ID: MGI:5823262
• Synonyms: RBE^KO
• Gene: Ctbp2 Location: Chr7:132589292-132726083 bp, - strand Genetic Position: Chr7, 76.32 cM
• Alliance: Ctbp2^tm1.2Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233453
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac x C57BL/6J

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: The endogenous N-terminal Ribeye A-domain was replaced with a loxP site followed by the Ribeye A domain cDNA fused to GCaMP3 (a GFP based calcium sensor). The coding region is followed by a polyA signal, an FRT-flanked PGK-neomycin selection cassette, and a 3' prime loxP site. The neo cassette is subsequently removed through flp-mediated recombination. The floxed tagged A-domain was deleted through cre-mediated recombination. This removes the possibility of transcribing the Ribeye isoform but leaves the other isoforms intact. (J:233453)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctbp2 Mutation: 572 strains or lines available

References

• Original: J:233453 Maxeiner S, et al., How to make a synaptic ribbon: RIBEYE deletion abolishes ribbons in retinal synapses and disrupts neurotransmitter release. EMBO J. 2016 May 17;35(10):1098-114
• All: 7 reference(s)