Plin5^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Plin5^{tm1c(KOMP)Wtsi}
• Name: perilipin 5; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5819278
• Synonyms: Plin5^fl
• Gene: Plin5 Location: Chr17:56418601-56424596 bp, - strand Genetic Position: Chr17, 29.16 cM
• Alliance: Plin5^{tm1c(KOMP)Wtsi} page
• IMPC: Plin5 gene page

Mutation origin

• Mutant Cell Line: EPD0301_4_E04
• Germline Transmission: Earliest citation of germline transmission: J:237392
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 56423013 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:237392)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plin5 Mutation: 25 strains or lines available

References

• Original: J:237392 Mohktar RA, et al., Perilipin 5 is dispensable for normal substrate metabolism and in the adaptation of skeletal muscle to exercise training. Am J Physiol Endocrinol Metab. 2016 Jul 01;311(1):E128-37
• All: 3 reference(s)