Igs2tm1(CAG-Myc*T58A/luc)Wrey
Targeted Allele Detail
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Symbol: |
Igs2tm1(CAG-Myc*T58A/luc)Wrey |
Name: |
intergenic site 2; targeted mutation 1, Robert Wechsler-Reya |
MGI ID: |
MGI:5812580 |
Synonyms: |
H11lox-STOP-lox -MycT58A, Igs2tm1(CAG-Myc*T58A/luc)Olvr, LSL-MycT58A |
Gene: |
Igs2 Location: unknown Genetic Position: Chr11, Syntenic
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Alliance: |
Igs2tm1(CAG-Myc*T58A/luc)Wrey page
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Allele Type: |
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Targeted (Conditional ready, Inserted expressed sequence, Reporter) |
Mutations: |
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Insertion, Nucleotide substitutions
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Igs2tm1(CAG-Myc*T58A/luc)Wrey expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Note |
mouse |
Myc (MGI:97250) |
contains a T58A substitution |
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Mutation details: The construct is designed to contain (from 5'to 3') an attB site, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked stop cassette (LSL), a myelocytomatosis oncogene (Myc) cDNA with a T58A mutation fused to an internal ribosome entry site (IRES) followed by a luciferase gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), and a polyadenylation sequence. This construct was microinjected, along with the PhiC31 integrase mRNA, into the pronuclei of zygotes collected from the TARGATT mice on a FVB/N congenic background. TARGATT mice carry the H11 allele ( Igs2tm3.1Luo) or H11P3; a unique sequence from the promoter of the yeast his3 gene followed by three shortened tandem attP sites and one FRT5 site. One founder mouse carried the correctly targeted transgenic insertion of the construct into the first and second attP sites of the locus.
(J:101977)
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
11 reference(s) |
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