Eif5a2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Eif5a2^{tm1c(EUCOMM)Hmgu}
• Name: eukaryotic translation initiation factor 5A2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5812101
• Synonyms: Eif5a2^p
• Gene: Eif5a2 Location: Chr3:28835426-28852995 bp, + strand Genetic Position: Chr3, 12.25 cM
• Alliance: Eif5a2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Eif5a2 gene page

Mutation origin

• Mutant Cell Line: HEPD0734_3_A07
• Germline Transmission: Earliest citation of germline transmission: J:236582
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 28835725 of Chromosome 3 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 3 at position 28837098. The exons 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exons 2 and 3. (J:236582)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eif5a2 Mutation: 11 strains or lines available

References

• Original: J:236582 Pallmann N, et al., Biological Relevance and Therapeutic Potential of the Hypusine Modification System. J Biol Chem. 2015 Jul 24;290(30):18343-60
• All: 1 reference(s)