Ddr2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ddr2^{tm1c(EUCOMM)Wtsi}
• Name: discoidin domain receptor family, member 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5806885
• Synonyms: Ddr2^fl
• Gene: Ddr2 Location: Chr1:169799876-169938331 bp, - strand Genetic Position: Chr1, 76.84 cM, cytoband H1-H5
• Alliance: Ddr2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ddr2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236031
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 169825258 of Chromosome 1 upstream of exon 8 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 8 at position 169826185. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon 8. (J:236031)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ddr2 Mutation: 60 strains or lines available

References

• Original: J:236031 Corsa CA, et al., The Action of Discoidin Domain Receptor 2 in Basal Tumor Cells and Stromal Cancer-Associated Fibroblasts Is Critical for Breast Cancer Metastasis. Cell Rep. 2016 Jun 14;15(11):2510-23
• All: 9 reference(s)