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Prmt8tm1d(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Prmt8tm1d(EUCOMM)Wtsi
Name: protein arginine N-methyltransferase 8; targeted mutation 1d, Wellcome Trust Sanger Institute
MGI ID: MGI:5806495
Synonyms: prmt8-
Gene: Prmt8  Location: Chr6:127689009-127769467 bp, - strand  Genetic Position: Chr6, 62.49 cM
Mutation
origin
Mutant Cell Line:  EPD0105_1_A03
Germline Transmission:  Earliest citation of germline transmission: J:234033
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 127712375 of Chromosome 6 upstream of exon 5 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 127711543. A knockout allele was created by flp and cre recombinase expression in mice, removing exon 5, the lacZ sequence and neomycin selection cassette. qRT-PCR analysis indicated that mRNA expression in the cerebellum was decreased by 45% in heterozygous and by >95% in homozygous mutant mice relative to wild-type controls. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:234033)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Prmt8 Mutation:  17 strains or lines available
References
Original:  J:234033 Kim JD, et al., PRMT8 as a phospholipase regulates Purkinje cell dendritic arborization and motor coordination. Sci Adv. 2015 Dec;1(11):e1500615
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/26/2020
MGI 6.15
The Jackson Laboratory