Top1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Top1^{tm1c(KOMP)Wtsi}
• Name: topoisomerase (DNA) I; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5805889
• Gene: Top1 Location: Chr2:160487901-160564684 bp, + strand Genetic Position: Chr2, 80.96 cM, cytoband H2-3
• Alliance: Top1^{tm1c(KOMP)Wtsi} page
• IMPC: Top1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236045
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 160511599 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 160512356. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ sequences and neomycin selection cassette. The critical exon(s) is/are remain flanked by loxP sites. (J:236045)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Top1 Mutation: 213 strains or lines available

References

• Original: J:236045 Mabb AM, et al., Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms. PLoS One. 2016;11(5):e0156439
• All: 3 reference(s)