Tg(Sox10-icre/ERT2)1Ldim
Transgene Detail

Summary

• Symbol: Tg(Sox10-icre/ERT2)1Ldim
• Name: transgene insertion 1, Leda Dimou
• MGI ID: MGI:5790496
• Synonyms: 1-Sox10
• Transgene: Tg(Sox10-icre/ERT2)1Ldim Location: unknown
• Alliance: Tg(Sox10-icre/ERT2)1Ldim page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Sox10-icre/ERT2)1Ldim expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sox10 (MGI:98358)

• Mutation details: The bacterial artificial chromosome (BAC) RP23-28011 containing the Sox10 locus was modified to construct a transgene. A targeting vector containing the sequence for a codon improved cre recombinase (icre) fused cDNA encoding mutated estrogen receptor (ERT2), SV40 polyA sequence and an Frt-flanked PGK-neo cassette replaced exon 3 of the Sox10 gene in the BAC by homologous recombination in bacterial cells. Arabinose-induced Flpe expression removed the neo cassette. The purified transgene was microinjected into C57BL/6 oocyte pronuclei. Two founder lines, 1-Sox10 and 2-Sox10, were obtained. Line 1 was determined to have 2 copies of the transgene, and line 2, which showed higher expression, contained 9 copies. (J:185481)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sox10 (mouse) Summary of all recombinase alleles driven by Sox10. Alliance mouse Sox10 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:185481 Simon C, et al., Sox10-iCreER(T2) : A mouse line to inducibly trace the neural crest and oligodendrocyte lineage. Genesis. 2012 Jun;50(6):506-15
• All: 4 reference(s)