Chrna5^tm1.1Itl
Targeted Allele Detail

Summary

• Symbol: Chrna5^tm1.1Itl
• Name: cholinergic receptor, nicotinic, alpha polypeptide 5; targeted mutation 1.1, inGenious Targeting Laboratory
• MGI ID: MGI:5767316
• Synonyms: alpha5DN
• Gene: Chrna5 Location: Chr9:54888164-54915063 bp, + strand Genetic Position: Chr9, 29.84 cM
• Alliance: Chrna5^tm1.1Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225451
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Dominant negative)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A G to A point mutation results in the amino acid substitution of asparagine for aspartic acid at position 397 (D397N). This mutation results in a dominant negative. The neomycin resistance cassette was removed. This nonsynonymous engineered SNP is equivalent to the human SNP rs16969968 (J:225451)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chrna5 Mutation: 40 strains or lines available

References

• Original: J:225451 Sciaccaluga M, et al., Crucial role of nicotinic alpha5 subunit variants for Ca2+ fluxes in ventral midbrain neurons. FASEB J. 2015 Aug;29(8):3389-98
• All: 2 reference(s)