Psmb11^em#Hasu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Psmb11^em#Hasu
• Name: proteasome (prosome, macropain) subunit, beta type, 11; endonuclease-mediated mutation, Harumi Suzuki
• MGI ID: MGI:5763138
• Synonyms: Psmb11^indel
• Gene: Psmb11 Location: Chr14:54862767-54867013 bp, + strand Genetic Position: Chr14, 27.87 cM
• Alliance: Psmb11^em#Hasu page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated
• Mutations: Insertion, Intragenic deletion, Single point mutation
• Mutation details: CRISPR technology was used to generate a G to A nucleotide substitution that results in the amino acid substitution of arginine for glycine at position 220 (G220R). Founder 2 is heterozygous for the expected point mutation without any additional mutations. Founder 3 is heterozygous for the expected point mutation and an additional single base pair insertion. Founder 21 is heterozygous for the expected point mutation and an additional 10 bp deletion. Founder 22 is heterozygous for the expected point mutation and an additional 8 bp deletion. Founder 28 is heterozygous for the expected point mutation without any additional mutations. The pound symbol (#) is used for the pool of founders 3, 21 and 22. (J:222872)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psmb11 Mutation: 13 strains or lines available

Notes

Guide RNA and hCas9 mRNA were injected into fertilized eggs from either (C57BL/6 x DBA/2)F1 x C57BL/6-Psmb11^Tn or C57BL/6 x C57BL/6-Psmb11^Tn.

References

• Original: J:222872 Nitta T, et al., The thymic cortical epithelium determines the TCR repertoire of IL-17-producing gammadeltaT cells. EMBO Rep. 2015 May;16(5):638-53
• All: 1 reference(s)