Rasa3^tm1.1Wber
Targeted Allele Detail

Summary

• Symbol: Rasa3^tm1.1Wber
• Name: RAS p21 protein activator 3; targeted mutation 1.1, Wolfgang Bergmeier
• MGI ID: MGI:5756029
• Synonyms: Rasa3^fl
• Gene: Rasa3 Location: Chr8:13617218-13727590 bp, - strand Genetic Position: Chr8, 6.29 cM
• Alliance: Rasa3^tm1.1Wber page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221899
• Parent Cell Line: PRX-B6N (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The HTGRS1006_A_4_H03 targeting vector was obtained from The International Knockout Mouse Consortium by The Jackson Laboratory. The L1L2_Pgk_P cassette was inserted at position 13615101 of Chromosome 8 upstream of the critical exon 3 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 13614300. The critical exon 3 is thus flanked by loxP sites. Flp-mediated recombination removed the reporter cassette and left exon 3 floxed. (J:221899)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rasa3 Mutation: 62 strains or lines available

References

• Original: J:221899 Stefanini L, et al., RASA3 is a critical inhibitor of RAP1-dependent platelet activation. J Clin Invest. 2015 Apr;125(4):1419-32
• All: 5 reference(s)