Tg(APOE-LIPC)357Hdic
Transgene Detail

Summary

• Symbol: Tg(APOE-LIPC)357Hdic
• Name: transgene insertion 357, Helen Dichek
• MGI ID: MGI:5750868
• Synonyms: hHL transgene
• Transgene: Tg(APOE-LIPC)357Hdic Location: unknown
• Alliance: Tg(APOE-LIPC)357Hdic page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(APOE-LIPC)357Hdic expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  LIPC (3990)

  Lipc	(MGI:96216)

• Mutation details: Mice with this transgene express wild-type human hepatic lipase (LIPC) under control of the regulatory elements of the human apolipoprotein E (APOE) locus. The construct contains 3 kb of 5'-flanking sequence, exon 1, intron 1, and the first 6 nucleotides of exon 2 from the human APOE gene followed by the cDNA encoding human LIPC and, from the human APOE locus, the untranslated region of exon 4, 0.1 kb of 3'-flanking sequence, and the first hepatic control region. Western blot analysis using a rabbit polyclonal anti-human LIPC antiserum confirmed the presence of immunoreactive human hepatic lipase in sera of mice bearing this transgene. Analysis of postheparin plasma demonstrated hepatic lipase activity in the human physiologic range in transgenic mice also homozygous for the null Lipc^tm1Unc mutation. (J:229079, J:229080)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:229079 Chen J, et al., In vivo structure-function studies of human hepatic lipase: the catalytic function rescues the lean phenotype of HL-deficient (hl-/-) mice. Physiol Rep. 2015 Apr;3(4)
• All: 2 reference(s)