Chd7^{tm2d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Chd7^{tm2d(EUCOMM)Wtsi}
• Name: chromodomain helicase DNA binding protein 7; targeted mutation 2d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5749387
• Gene: Chd7 Location: Chr4:8690406-8867659 bp, + strand Genetic Position: Chr4, 3.68 cM
• Alliance: Chd7^{tm2d(EUCOMM)Wtsi} page
• IMPC: Chd7 gene page

Mutation origin

• Mutant Cell Line: EPD0019_1_D07
• Germline Transmission: Earliest citation of germline transmission: J:222062
• Parent Cell Line: JM8 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_st0
• Mutation details: The L1L2_st0 cassette was inserted at position 8784907 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 3 at position 8786002. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:222062)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chd7 Mutation: 134 strains or lines available

References

• Original: J:222062 Jones KM, et al., CHD7 maintains neural stem cell quiescence and prevents premature stem cell depletion in the adult hippocampus. Stem Cells. 2015 Jan;33(1):196-210
• All: 1 reference(s)