Col1a1^{tm3(CAG-Tn,-DsRed)Fcam}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm3(CAG-Tn,-DsRed)Fcam}
• Name: collagen, type I, alpha 1; targeted mutation 3, Fernando D Camargo
• MGI ID: MGI:5703070
• Synonyms: Tn
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm3(CAG-Tn,-DsRed)Fcam} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217487
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Reporter, Transposon concatemer)
• Mutation: Insertion
• Mutation details: A construct consisting of a CAG promoter, a single copy of a hyperactive Sleeping Beauty responsive transposon-stop cassette and a DsRed cassette was inserted by RMCE using ES cells carrying Col1a1^{tm13(neo/hygro*)Jae}. Hyperactive Sleeping Beauty mediated transposition of the transposon results in expression of the reporter. (J:217487)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm2(tetO-Pou5f1)Jae} (J:159351).

References

• Original: J:217487 Sun J, et al., Clonal dynamics of native haematopoiesis. Nature. 2014 Oct 16;514(7522):322-7
• All: 2 reference(s)